PRIMER PICKING RESULTS FOR TIMP4-E1-E2

No mispriming library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3' seq
LEFT PRIMER        616   19   60.00   57.89  3.00  2.00 CACCCTCAGCAGCACATCT
RIGHT PRIMER       738   20   59.11   40.00  4.00  0.00 TTTGATTTCATACCGGAGCA
SEQUENCE SIZE: 748
INCLUDED REGION SIZE: 160

PRODUCT SIZE: 123, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 660,2

    0 GCTCAGCAAGGGGTCCGTCCTTCTCTGTCACTGTCTCTTTTGCCTGTTGTAATTCTGTCT
      ............................................................

   60 GCCTCTCTGGGACTCTGCCTGTCTCACTCTTTCTGTCTGTGCCTCTCCTCACTCTTGTTC
      ............................................................

  120 TTTCTGCCTGAATCACAGCCCTCAGTTTTTCTGTCCTCATGCATTTGTCTTTGTGGCTCT
      ............................................................

  180 TTCCGTCTTTCTGCCCTTGACACCATCCCCTCTCCCAGTGCTTCCCCTCTGCTTCCAGAT
      ............................................................

  240 CGCTTCATGACTTAGGCAGGGAAACAGAGGTCAGGGCCTCCTTCCAGGCTTCCCTCTGCA
      ............................................................

  300 TCTTACTGAGTATGCAGGTCGGAAGAGCCTCGGGTCCTGCCTCCGCGGGTGGCCTAGAGC
      ............................................................

  360 CAAAGGAAGGCGGAGCCCGTCGGGGCGGGATTGGCCCTTAGGGCCACCTCATAAAGCCTG
      ............................................................

  420 GGGCGAGGGGCACAACGGCCTTGGGAAGGAGCCCTGCTGGGGCCGTCCAGTCCCCCAGAC
      ............................................................

  480 CTCACAGGCTCAGTCGCGGATCTGCAGTGTCATGCCTGGGAGCCCTCGGCCCGCGCCAAG
      ............................................................

  540 CTGGGTGCTGTTGCTGCGGCTGCTGGCGTTGCTGCGGCCCCCGGGGCTGGGTGAGGCATG
      ........................................                    

  600 CAGCTGCGCCCCGGCGCACCCTCAGCAGCACATCTGCCACTCGGCACTTGTGATTCGGGC
                      >>>>>>>>>>>>>>>>>>>                         

  660 CAAAATCTCCAGTGAGAAGGTAGTTCCGGCCAGTGCAGACCCTGCTGACACTGAAAAAAT
      **                                                         <

  720 GCTCCGGTATGAAATCAAACAGATAAAG
      <<<<<<<<<<<<<<<<<<< ........

KEYS (in order of precedence):
...... vector sequence
****** target
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
                    start  len      tm     gc%   any    3' seq

 1 LEFT PRIMER        638   19   59.85   52.63  4.00  2.00 ACTCGGCACTTGTGATTCG
   RIGHT PRIMER       738   20   59.11   40.00  4.00  0.00 TTTGATTTCATACCGGAGCA
   PRODUCT SIZE: 101, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER        623   19   60.16   57.89  6.00  2.00 AGCAGCACATCTGCCACTC
   RIGHT PRIMER       738   20   59.11   40.00  4.00  0.00 TTTGATTTCATACCGGAGCA
   PRODUCT SIZE: 116, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        616   19   60.00   57.89  3.00  2.00 CACCCTCAGCAGCACATCT
   RIGHT PRIMER       739   21   59.95   42.86  6.00  0.00 GTTTGATTTCATACCGGAGCA
   PRODUCT SIZE: 124, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        616   19   60.00   57.89  3.00  2.00 CACCCTCAGCAGCACATCT
   RIGHT PRIMER       716   21   59.90   47.62  3.00  1.00 TTTTCAGTGTCAGCAGGGTCT
   PRODUCT SIZE: 101, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00


